CHROMATOGRAPHY
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Chromatography Specifications
CHROMAFLEX® Columns CHROMAFLEX® Chromatography Columns have a unique design feature: PTFE shielded o-rings. Each end fitting has a thin PTFE layer that is curled over the o-ring to form a solvent resistant, leak free seal. CHROMAFLEX® Columns can be used with all aqueous buffers without modification and with organic solvents when the optional PTFE bed supports are installed in the end fittings and flow adapters. CHROMAFLEX® Columns are available in both standard and water jacketed versions. Standard CHROMAFLEX® Columns Standard CHROMAFLEX® Columns are available in three IDs (1.0, 2.5 and 4.8 cm) in five standard lengths (15, 30, 60, 100 and 120 cm). Each column is supplied with two PTFE end fittings with 20 µm porosity polyethylene bed supports, 5 feet PTFE tubing (1/16” OD tubing with 1.0 cm, 1/8” OD tubing with 2.5 & 4.8 cm), 2 flangeless tubing nuts and 2 flangeless ferrules. Jacketed CHROMAFLEX® Columns Jacketed CHROMAFLEX® Columns are provided with an acrylic water jacket to provide thermal control of temperature sensitive chromatographic separations. The acrylic jacket also acts as a safety shield. Jacketed columns are available in all of the standard CHROMAFLEX® Column sizes and supplied with the same tubing, nuts and ferrules. Flow Adapters CHROMAFLEX® Flow Adapters allow easy adjustment of the column bed volume. They are ideal for concentration and pH gradients, reverse flow separations, and gravity flow separations that require a high level of resolution and reproducibility. Flow adapters are available for all three standard ID columns. Packing Reservoirs The CHROMAFLEX® Glass Reservoirs are ideal for packing gel slurries into columns and as buffer reservoirs for simple gravity flow chromatography. Each reservoir has been designed for easy mounting on all CHROMAFLEX® Columns. The reservoir capacities are matched by column ID to take the amount of slurry required to pack the longest standard column. The reservoir end fitting has 1/4-28 threads for simple flangeless connection to a pump. Flangeless 1/4-28 Fittings All tubing connections to CHROMAFLEX® Columns can be made with easy-to-use 1/4-28 flangeless fittings and either 1/16” OD, 1/8” OD or 1.8 mm OD tubing. Our complete line of fittings and adapters allow simple connection to any chromatography system. Custom Length Columns Certain applications may require a column length not found in our standard product offering. Please contact customer service for pricing and delivery details for custom length CHROMAFLEX® Columns. Specifications
Materials Column Barrels:
Type I, Class A Borosilicate Glass Polytetrafluoroethylene (PTFE) High Density Polyethylene (HDPE) Acrylic (jacketed columns only) Polypropylene (jacketed columns only)
420870-3010 jacketed column shown with optional 420876-0020 flow adapter.
End Fitting: Bed Support:
Water/Safety Jacket: Jacket Sealing Rings: Bed Support Porosity: Maximum Pressure*:
20 micron nominal (for standard bed support) 1.0 cm ID Columns - 100 psi (6.9 bar) 2.5 cm ID Columns 75 psi (5.2 bar) 4.8 cm ID Columns - 50 psi (3.4 bar) Temperature Range: 0 to 50 °C
Tubing Connections:
1/4-28 flangeless fittings
Recommended: Ethylene Oxide, 2N NaOH or 100% Ethanol Sterilization Method:(autoclaving is not recommended)
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