CHROMATOGRAPHY
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Tubing / FLEX-COLUMN® Economy Columns
Chromatography Tubing Even if you thoroughly degas your mobile phase by helium sparging , vacuum filtration , sonication or in-line vacuum degassing , the mobile phase will not stay degassed if PTFE tubing is used to deliver it to the pump. That’s because PTFE is gas-permeable and allows ambient gases, such as oxygen, to diffuse through the tubing wall and into the mobile phase. The amount of ambient gas that enters the mobile phase depends on the tubing dimensions and mobile phase flowrate, but no matter how well you have degassed, some oxygen will re-enter the mobile phase on its way to the pump, possibly detracting from system performance. NO-OX tubing solves this problem with a unique double-wall design that eliminates regassing .
• Chemically inert inner FEP PTFE tube carries the mobile phases • Non-wetted outer wall is made from a translucent, flexible polymer with extremely low gas permeability • Cuts regassing rates to negligible levels while preserving PTFE’s excellent chemical resistance, transparency and handling properties • Bends easily to fit your system’s layout and cuts easily with a razor blade or plastic tubing cutter • Translucent wall lets you see the tubing’s interior for fast, simple troubleshooting • NO-OX fitting connects in seconds • H90669 is supplied with an integral male luer lock for easy connection to Flex-Columns® Part Number Tube OD (in) 420823-0116 0.0625
Tube Size (ft)
Case Qty
10 10 11
1 1 1 5
420823-0018 420823-0014 H90669-0005
0.125
0.25
Simply slide the flangeless nut and ferrule over the tubing, push the NO-OX insert into the tubing end and connect finger-tight into any flat bottom 1/4-28 threaded port.
0.0625
5
Luer Lock Fitting
FLEX-COLUMNS® are economical, easy-to-use chromatography columns that are ideal for use with gel filtration, ion exchange, affinity and adsorption media. The columns are constructed of polypropylene reservoirs and column outlets permanently connected to a borosilicate glass barrel. The 20 µm porosity polyethylene bed supports are fixed in the column outlets. Luer lock inlets and outlets on the columns, along with a wide variety of adapters, valves and fittings provide simple and versatile tubing connections. Standard Columns Standard FLEX-COLUMNS® are available in 30 different sizes with standard inside diameters of 0.7, 1.0, 1.5 and 2.5 cm and lengths ranging from 4 to 120 cm. All columns are fitted with translucent polypropylene reservoir tops, and bed supports that are permanently mounted in the polypropylene column outlet. Short columns are typically used for ion exchange chromatography, sample desalting and semidisposable applications, while the long columns are ideally suited for gel filtration and adsorption chromatography. Flow Adapters FLEX-COLUMN® Flow Adapters allow easy adjustment of the column bed volume. They are ideal for concentration and pH gradients, reverse flow separations, and gravity flow separations that require a high level of resolution and reproducibility. Flow adapters are available for 1.0, 1.5 and 2.5 cm ID columns. Luer Lock Fittings The male luer lock fittings on all FLEX-COLUMNS® along with our complete line of fittings, valves and adapters allow easy connection to any low pressure chromatography system. Custom Length Columns Certain applications may require a column length not found in our standard product offering. Please contact Customer Service for pricing and delivery details for custom length FLEX-COLUMNS®. Call 888-546-2531.
Threaded Reservoir Cap
Translucent Reservoir Top
Borosilicate Glass Column
Precision Ground Column Ends
Specifications
Materials:
Column Barrels: Type I, Class A Borosilicate Glass
Cap, Reservoir & Column Outlet: Polypropylene
Bed Support: High Density Polyethylene (HDPE) Bed Support Porosity: 20 micron nominal Maximum Pressure: 1.0 bar (14.7 psi, 1.0 atm, 0.1 MPa) Temperature Range: 0 to 50 °C Tubing Connections: Male Luer Locks Recommended: 2N NaOH or 100% Ethanol Sterilization Method: (Autoclaving is not recommended.)
Note: Polypropylene end fittings are NOT replaceable on FLEX-COLUMNS®.
Luer Lock Column Outlet
20 Micron Polyethylene Bed Support
* CAUTION Pressurized glassware should always be used with adequate safety shielding.
420400
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