CHROMATOGRAPHY
81
Economy Flow Adapters / Packing Reservoirs / Diffusion Disc
FLEX-COLUMN® Economy Flow Adapter Flow adapters can significantly improve FLEX- COLUMN® performance by protecting the gel bed from disruption during sample loading and by eliminating any dead volume above the gel bed. They are ideal for concentration and pH gradients, reverse flow separations and gravity flow separations that require a high level of resolution and reproducibility.
FLEX-COLUMN® Packing Reservoirs These funnels are used for initial column packing and as buffer reservoirs for simple gravity flow chromatography.
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Manufactured from polypropylene and available in two capacities: 100 mL for the 0.7, 1.0 and 1.5 cm ID columns and 700 mL for the 2.5 cm columns
• Manufactured from 33 expansion, low extractable borosilicate glass conforming to USP Type I and ASTM E438, Type I, Class A requirements
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Flow adapters improve chromatogram resolution by delivering the sample and buffer directly to the top of the gel bed Flow adapters are also recommended when using buffer gradients or when the columns are connected to a low pressure pump system Flow adapters consist of 1/16” OD FEP PTFE tubing, a polyacetal body, a FKM o-ring seal and a 20 μm porosity HDPE bed support One replacement bed support is supplied with each flow adapter Flow adapters are available for 1.0, 1.5 and 2.5 cm ID columns
Used for Column ID (cm)
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Part Number
Capacity (mL)
Case Qty
420405-0100 420405-0700
100 700
12
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2.5
5
FLEX-COLUMN® Sample Diffusion Disc • Sample diffusion discs protect the gel bed from being disrupted during sample application whenever a flow adapter is not used • A transparent acrylic ring is sealed to a 20 μm porosity HDPE disc which protects the top of the gel bed when the sample is manually applied • A crossbar on the top of the acrylic ring permits easy removal of the sample diffusion disc from the column Part Number Fits Column ID (cm) Case Qty 420404-1000 1 1 420404-1500 1.5 1 420404-2500 2.5 1
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Overall Length (mm)
Part Number Fits Column ID (cm)
Case Qty
420415-1000 420415-1500 420415-2500
1
N/A N/A N/A
1 1 1
1.5 2.5
Replacement Parts
Part Number
Description
Case Qty
1.0 cm, 20 micron Porosity HDPE Bed Support 1.5 cm, 20 micron Porosity HDPE Bed Support 2.5 cm, 20 micron Porosity HDPE Bed Support Polypropylene Ferrule for 1/8” OD Tubing, Fits Columns ID 2.5/4.8 1/16” OD x 0.038” ID x 10’ FEP / PTFE Tubing, Fits 20 Gauge Needle
420415-1001
10
420415-1501
10
420415-2501
10
420828-0116
1
420823-0116
1
Column with Flow Adapter
Column with Diffusion Disc
420408
H90669
420407
420407
420163
420163
420163
420818
420822
420823
420822
420821
H90669
420407
420407
420821
420823
420818
420822 420821
420823
420822
TYPICAL ASSEMBLIES
420821 420823
420823
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