[DWK Life Sciences] Product Overview

53 Cell Culture

CELLine ™ Bioreactors Our CELLine flasks are designed to enhance small scale bio-production for antibody and protein generation. Conventional in vivo or in vitro cell culture methods can be laborious, may have low cell density and require significant purification. CELLine flasks address these three areas of limitation observed in static tissue culture flasks.

How does the CELLine Flask Work? Media Compartment – The media compartment allows for bulk storage of cell culture growth medium. This reduces the media refreshing requirement significantly as the media compartment is fifty times the size of the cell compartment. Metabolite Regulating Upper Membrane – The upper dialysis membrane has a 10 kDa cut off limit. This regulates the flow of metabolites to and from the cell compartment and retains all proteins in the cell compartment. Cell Compartment – The cell compartment provides the ideal area to inoculate and achieve high density cultures. The compartment concentrates cells, their products, and limits the requirement for any exogenous growth factors. Gas Permeable Lower Membrane – With static cultures, gas transfer rates can be the limiting factor in high density cultures. The CELLine flask places the cells directly against the gas permeable membrane to achieve optimal levels of oxygen and carbon dioxide.

Nutrients Glucose Glutamine

Waste Products Lactate Ammonium

Inoculation / Harvest Port

Media Port

Media Compartment

10kDa Membrane

Pipette Tip Stop

Inoculation / Harvest Tube

Cells

Cell Compartment

Cell Compartment Spacer

Metabolite Regulating Upper Membrane

Proteins

Silicone Membrane

Gas Permeable Lower Membrane

O 2

CO 2

Base

Note: Exploded view of device; unit is packaged fully assembled

Culture

Media (mL)

Cell (mL)

Qty /

Cat. No.

Flask Type

Type

Compartment Size

Case

WCL1000-1 WCL1000-3

CELLine 1000 CELLine 1000

Suspension Suspension

1000 1000 1000 1000

15 15 15 15

1 3 1 3 1 5

WCL1000AD-1 WCL1000AD-3

CELLine 1000-AD CELLine 1000-AD

Adherent Adherent

WCL0350-1 WCL0350-5

CELLine 350 CELLine 350

Suspension Suspension

350 350

5 5

Benefits of CELLine Flask • Disposable and ready-to-use • High cell density and high product concentration • Reduces operation time • Decreases use of consumables • Cost-efficient, space saving, and stackable • No additional equipment required for operation