Chromo-LAL
Stability Once reconstituted, Chromo-LAL is stable for 24 hours if stored at 2–8°C. Chromo-LAL may be frozen once and will retain activity for 2 weeks if stored at or below -20°C. Packaging Each vial contains reagent for approximately 30 tests. It is recommended for use with 0.5 μg/vial Control Standard Endotoxin (CSE, E0005-1). Certificates of Analysis, specific to the Chromo-LAL and CSE lot, can be obtained from ACC or online at www.acciusa.com.
Chromo-LAL #C0031-5 3.2 mL/Vial 5 Pack (150 tests)
Chromo-LAL is optimized for the kinetic chromogenic BET test method in microplate readers. Chromo-LAL is a buffered, stable and robust lysate, suitable for quan- titative testing of a wide range of samples. Sensitivity The sensitivity for chromogenic assays is determined by the lowest standard concentration on the standard curve used for the assay. The maximum sensitivity of Chromo-LAL is 0.005 EU/mL. Sample to BET Ratio Reconstituted Chromo-LAL reagent is used at a ratio of 1:1 and a volume of 100 μL/well (30 tests/vial). Performing the Test The Chromo-LAL/sample mixture is incubated at 37±1°C and read in a microplate reader. Software is used to construct the standard curve and calculate the endotoxin concentrations. Reconstitution Chromo-LAL lysate is reconstituted with BET Reagent Water (LRW). It can also be reconstituted with Gluca- shield ® buffer, a (1 g 3)- b - D -Glucan inhibiting buffer, to render the assay endotoxin specific.
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